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Synthetic Biology |
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Markwalter CE, Uralcan B, Pelczer I, Zarzhitsky S, Hecht MH, Prud’homme RK, Debenedetti PG (2020) Stability of Protein Structure during Nanocarrier Encapsulation: Insights on Solvent Effects from Simulations and Spectroscopic Analysis. ACS nano, 14(12), 16962-72. |
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Wang MS and Hecht MH (2020) A Completely De Novo ATPase from Combinatorial Protein Design. J. Am. Chem. Soc. 142(36), 15230-15234. |
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Mancini JA, Pike DH, Tyrishkin AM, Haramaty L, Wang MS, Poudel S, Hecht MH, Nanda V (2020) Design of a Fe4S4 Cluster into the Core of a De Novo 4‐Helix Bundle. Biotechnology and Applied Biochemistry 67, 574-85. |
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Zarzhitsky S, Jiang A, Stanley E, Hecht MH (2020) Harnessing Synthetic Biology to Enhance Heterologous Protein Expression. Protein Science 29(8), 1698-1706. |
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Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R (2020) Hyperstable De Novo Protein with a Dimeric Bisecting Topology. ACS Synthetic Biology 9(2), 254-9. |
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Karas C, Hecht MH (2020) A Strategy for Combinatorial Cavity Design in De Novo Proteins. Life 10(2), 9. |
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Wang MS, Hoegler K, Hecht MH (2019) Unevolved De Novo Proteins Have Innate Tendencies to Bind Transition Metals. Life 9, 8. |
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Hecht MH, Zarzhitsky S, Karas C, Chari S (2018) Are Natural Proteins Special? Can We Do That? Current Opinion in Structural Biology 48, 124-132. |
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Donnelly AE, Murphy GS, Digianantonio KM, Hecht MH (2018) A De Novo Enzyme Catalyzes a Life-Sustaining Reaction in E. coli. Nature Chemical Biology 14, 253–255. |
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Hoegler KJ, Hecht MH (2018) Artificial Gene Amplification in Escherichia Coli Reveals Numerous Determinants Enabling Resistance to Metal Toxicity. J. Molecular Evolution 86, 103–110. |
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Kobayashi N, Inano K, Sasahara K, Sato T, Miyazawa K, Fukuma T, Hecht MH, Song C, Murata K, Arai R (2018) Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks. ACS Synthetic Biology 7 1381–1394. |
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Digianantonio KM, Korolev, M, Hecht MH (2017) A Non-Natural Protein Rescues Cells Deleted for a Key Enzyme in Central Metabolism. ACS Synthetic Biology 6, 694–700. |
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Digianantonio KM, Hecht MH (2016) A Protein Constructed De Novo Enables Cell Growth by Altering Gene Regulation Proc. Natl. Acad. Sci.(USA) 113, 2400–2405. |
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Murphy GS, Greisman JB, Hecht MH (2015) De Novo Proteins with Life-Sustaining Functions are Structurally Dynamic. J. Molec. Biology 428, 399-411. |
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Hoegler KJ, Hecht MH (2016) A De Novo Protein Confers Copper Resistance in Escherichia Coli. Protein Science 25, 1249-1259. |
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Kobayashi N, Yasase K, Sato T, Hecht MH, Arai R (2015) Self-Assembling Nano-Architectures Created from a Protein Nano-Building Block Using Domain-Swapped Dimeric De Novo Protein. J. Am. Chem. Soc. 137, 11285–11293. (Cover of JACS). |
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Smith BA, Mularz AM and MH Hecht (2014) Divergent evolution of a bifunctional de novo protein. Protein Science 24, 246-252. |
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Patel, SC & Hecht MH (2012) Directed Evolution of the Peroxidase Activity of a De Novo Designed Protein. Protein Engineering, Design& Selection (PEDS) (Cover Article) 25, 445–451. |
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Cherny I, Korolev M, Koehler AN and MH Hecht (2012)Proteins from an Unevolved Library of de novo Designed Sequences Bind a Range of Small Molecules. ACS Synthetic Biology 1, 130-138. |
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Arai R, Kobayashi N, Kimura A, Sato T, Matsuo K, Wang AF, Platt JM, Bradley LH, & Hecht MH (2012) Domain-Swapped Dimeric Structure of a Stable and Functional De Novo 4-Helix Bundle protein, WA20. Physical Chemistry B. 116, 6789–6797. |
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Smith BA & Hecht MH (2011) Functional de novo Proteins (Review). Current Opinion in Chemical Biology 15, 421–426. |
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Das A, Wei Y, Pelczer I & Hecht MH (2011) Binding of small molecules to cavity forming mutants of de novo designed protein. Protein Science 20,702-711. |
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Fisher MA, McKinley KL, Bradley LH, Viola SR & Hecht MH (2011) De Novo Designed Proteins From a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth. PLoS ONE 6(1), e15364. |
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Patel S, Bradley LH, Jinadasa S, Hecht MH. (2009) Cofactor Binding and Enzymatic Activity in an Unevolved Superfamily of De Novo Designed 4-Helix Bundle Proteins. Protein Science 18, 1388-1400. |
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Go A, Kim S, Baum J, & Hecht MH (2008) Structure and Dynamics of De novo Proteins from a Designed Superfamily of 4-Helix Bundles. Protein Science 17, 4. |
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Das A, Hecht MH (2007) Peroxidase Activity of De Novo Heme Proteins Immobilized on Electrodes. J. Inorganic Biochemistry 101, 1820-1826. |
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Das A, Trammell SA, & Hecht MH (2006) Electrochemical and ligand binding studies of a de novo heme protein. Biophysical Chemistry 123, 102-112. |
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Bradley LH, Thumfort P, Hecht MH. (2006) De Novo Proteins from Binary Patterned Combinatorial Libraries. Chapter 3 in Protein Design: Methods & Applications in Methods in Molecular Biology (Humana Press) 340, 53-69. |
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Hu Y, Das A, Hecht MH & Scoles G (2005) Nanografting De Novo Proteins onto Gold Surfaces. Langmuir 21, 9103-9109. |
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Bradley LH, Kleiner RE, Wang AF, Hecht MH & Wood DW (2005) An Intein-Based Genetic Selection Enables Construction of a High-Quality Library of Binary Patterned De Novo Sequences. Protein Engineering, Design and Selection 18, 201-207. |
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Klepeis JL, Wei Y, Hecht MH & Floudas CA (2004) Ab Initio Prediction of the 3-Dimensional Structure of a De Novo Designed Protein: A Double Blind Case Study. Proteins: Structure, Function, and Bioinformatics 58, 560-570. |
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Hecht MH, Das A, Go A, Bradley LH, Wei Y (2004) De Novo Proteins From Designed Combinatorial Libraries. Protein Science 13, 1711-1723. |
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Wei Y & Hecht MH (2004) Enzyme-like Proteins From an Unselected Library of Designed Amino Acid Sequences. Protein Engineering, Design and Selection 17, 67-75. |
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Wei Y, Kim S, Fela D, Baum J, & Hecht MH (2003) Solution Structure of a De Novo Protein From a Designed Combinatorial Library. Proc. Natl. Acad. Sci. (USA) 100, 13270-13273. |
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Moffet DA, Foley J, & Hecht MH (2003) Midpoint Reduction Potentials and Heme Binding Stoichiometries of De Novo Proteins from Designed Combinatorial Libraries. Biophysical Chemistry 105, 231-239. |
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Wei Y, Liu T, Sazinsky SL, Moffet DA, Pelczer I, & Hecht MH (2003) Stably Folded De Novo Proteins From a Designed Combinatorial Library. Protein Science 12, 92-102. |
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Wang W, & Hecht MH (2002) Rationally Designed Mutations Convert De Novo Amyloid-Like Fibrils into Soluble Monomeric β-Sheet Proteins. Proc. Natl Acad. Sci.(USA) 99, 2760-2765. |
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Brown CL, Aksay IA, Saville DA, & Hecht MH (2002) Template-Directed Assembly of a De Novo Designed Protein. J. Am. Chem. Soc. 124, 6846-6848. |
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Moffet DA & Hecht MH (2001) De Novo Proteins From Combinatorial Libraries. Chemical Reviews 101, 3191-3204. |
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Xu, G, Wang W, Groves JT, Hecht MH (2001) Self-Assembled Monolayers from a Designed Combinatorial Library of De Novo Beta-Sheet Proteins. Proc. Natl. Acad. Sci. (USA) 98, 3652-3657. |
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Moffet DA, Case MA, House JC, Vogel K, Williams R, Spiro TG, McLendon GL, Hecht MH (2001) Carbon Monoxide Binding by De Novo Heme Proteins From a Designed Combinatorial Library. J. Am. Chem. Soc. 123, 2109-2115. |
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Moffet DA, Certain LK, Smith AJ, Kessel AJ, Beckwith KA &Hecht MH (2000) Peroxidase Activity in Heme Proteins Derived From a Designed Combinatorial Library. J. Am. Chem. Soc. 122, 7612-7613. |
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Roy S & Hecht MH (2000) Cooperative Thermal Denaturation of Proteins Designed by Binary Patterning of Polar and Nonpolar Amino Acids. Biochemistry 39, 4603-4607. |
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Broome BM & Hecht MH (2000) Nature Disfavors Sequences of Alternating Polar and Nonpolar Amino Acids: Implications for Amyloidogenesis. J. Molecular Biology 296, 961-968. |
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West MW, Wang W, Patterson J, Mancias JD, Beasley JR & Hecht MH (1999) De Novo Amyloid Proteins From Designed Combinatorial Libraries. Proc. Natl. Acad. Sci. (USA) 96, 11211-11216. |
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Rosenbaum DM, Roy S, & Hecht MH (1999) Screening Combinatorial Libraries of De Novo Proteins By Hydrogen-Deuterium Exchange and Electrospray Mass Spectrometry. J. Am. Chem. Soc. 121, 9509-9513. |
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Rojas NR, Kamtekar S, Simons CT, McLean JE, Vogel KM, Spiro TG, Farid RS & Hecht MH (1997) De Novo Heme Proteins From Designed Combinatorial Libraries. Protein Science 6, 2512-2524. |
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West MW & Hecht MH (1995) Binary Patterning of Polar and Nonpolar Amino Acids in the Sequences and Structures of Native Proteins. Protein Science 4, 2032-2039. |
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Xiong H, Buckwalter BL, Shieh HM & Hecht MH (1995) Periodicity of Polar and Non-Polar Amino Acids is the Major Determinant of Secondary Structure in Self-Assembling Oligomeric Peptides. Proc. Natl. Acad. Sci. (USA) 92, 6349-6353. |
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Johnson BH & Hecht MH (1994) Recombinant Proteins Can Be Released From E. Coli Cells By Repeated Cycles of Freezing and Thawing. Biotechnology 12, 1357-1360. |
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Kamtekar S, Schiffer JM, Xiong H, Babik JM & Hecht MH (1993) Protein Design by Binary Patterning of Polar and Non-Polar Amino Acids. Science 262, 1680-1685. |
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Brunet AP, Huang ES, Huffine ME, Loeb JE, Weltman RJ & Hecht MH (1993) The Role of Turns in Dictating the Structure of an Alpha-Helical Protein. Nature 364, 355-358. |
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